Difference between revisions of "Production of Unilamellar Vesicles Using an Inverted Emulsion"

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==Summary==
 
==Summary==
  
The article presents a novel emulsification method to prepare unilamellar liposomes ranging between .1 <math>\mu</math>m to 1 <math>\mu</math>m.   
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The article presents a novel emulsification method to prepare unilamellar liposomes ranging between .1 <math>\mu</math>m to 1 <math>\mu</math>m.  Previous liposomal preparation techniques such as sonication, electroformation, rehydration of lipid films, extrusion, etc, do not allow simultaneous control of size, encapsulation yield, and lipid composition.  In contrast, the technique presented on this paper helps overcome some of these issues by forming vesicles independently one layer at a time.  First, water is emulsified in oil containing the desired lipid as surfactant.  Water drops stabilized at the surface by the lipids will compose the core and inner lipid layer of the vesicles.  Second, water is then put in a separate vial, and oil containing lipids is poured on top.  These lipids will constitute the outer layer of the vesicles.  The emulsified water is then added on top of the second vial containing the two layers of water and oil.  The emulsion droplets sink to the bottom passing through the lipid interface and in doing so, the outer layer of the vesicles form as seen in the figure below.
 
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==Soft Matter Connection==
 
==Soft Matter Connection==

Revision as of 01:23, 5 December 2009

(in progress...)

Weitz D.A. et al. Langmuir 2003, 19, 2870-2879

Keywords

Summary

The article presents a novel emulsification method to prepare unilamellar liposomes ranging between .1 <math>\mu</math>m to 1 <math>\mu</math>m. Previous liposomal preparation techniques such as sonication, electroformation, rehydration of lipid films, extrusion, etc, do not allow simultaneous control of size, encapsulation yield, and lipid composition. In contrast, the technique presented on this paper helps overcome some of these issues by forming vesicles independently one layer at a time. First, water is emulsified in oil containing the desired lipid as surfactant. Water drops stabilized at the surface by the lipids will compose the core and inner lipid layer of the vesicles. Second, water is then put in a separate vial, and oil containing lipids is poured on top. These lipids will constitute the outer layer of the vesicles. The emulsified water is then added on top of the second vial containing the two layers of water and oil. The emulsion droplets sink to the bottom passing through the lipid interface and in doing so, the outer layer of the vesicles form as seen in the figure below.

Soft Matter Connection