Microoxen: Microorganisms to Move Microscale Loads.
"Microoxen: Microorganisms to Move Microscale Loads"
Douglas B. Weibel, Piotr Garstecki, Declan Ryan, Willow R. DiLuzio, Michael Mayer, Jennifer E. Seto, & George M. Whitesides
PNAS 102(34) 11963-11967 (2005)
Soft Matter Keywords
algae, phototaxis, photochemistry, beast of burden
The authors detail a very novel approach to transporting small payloads using biological motors. Instead of attaching synthesized motors to a load, Weibel, et al. attach the load to an organism. This allows them to steer the transport of the load by controlling the locomotion of the organism. In this case, the unicellular photosynthetic algae Chlamydomonas reinhardtii was chosen for its robust locomotion, phototactic characteristics, and ease of culture. The simulated loads in these experiments were surface modified polystyrene beads. The peptide used to attach the bead to the algae cell contained a photo-active group that allows the bead to be cleaved from the cell when exposed to UV light of the appropriate wavelength. In this way, the beads can be delivered to a particular location and then released.
Practical Application of Research
This system works nicely for transporting microscale objects over relatively long distances (10s of centimeters). As Weibel, et al. point out, this system cannot be scaled down to the nanoscale, but does have the advantage of using an existing organism, which precludes engineering the control of biological motors attached directly to loads. One challenge that must be addressed before this is a fully viable system is controlled attachment of beads to the cells. An ideal system would allow precise placement of the bead on the cell surface so it doesn't impede locomotion.
Moving Loads with Tiny Oxen
Chlamydomonas reinhardtii (CR) is a type of photosynthetic algae that propels itself using two flagella. The flagella are approximates 12 microns in length and execute a breaststroke-like motion, as shown in Figure 1. The flagella beat at a frequency around 40-60 Hz and can propel the 10 micron diameter algae at velocities in the neighborhood of 100-200 microns/second. As the cells swim, they rotate counterclockwise above their longitudinal axis, tracing out a helical path. CR cells exhibit phototactic ability, with a maximum response at 505nm and a secondary response at 443nm. At high intensities, the cells exhibit negative phototaxis, swimming away from the light source, while at intermediate intensities, the cells are attracted to the light (positive phototaxis). Weibel, et al. find that attaching a polystyrene bead (1-6 microns in diameter) to the cell has little effect on the algae's locomotion. Only when the bead was attached near the flagella or the algae were swimming in confined channels such that the bead occasionally made contact with the channel walls, did the trajectory and velocity vary significantly from that of a cell swimming without a bead attached.
written by Donald Aubrecht