Difference between revisions of "Magneto-mechanical mixing and manipulation of picoliter volumes in vesicles"

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containing the superparamagnetic beads was added to the dried lipid. The two ITO plates were mounted in parallel and an electric field was applied. Finally, the voltage was increased to facilitate the separation
 
containing the superparamagnetic beads was added to the dried lipid. The two ITO plates were mounted in parallel and an electric field was applied. Finally, the voltage was increased to facilitate the separation
 
of vesicles.
 
of vesicles.
A theoretical minimum magnetic field of <math> 59 \mu T </math> is needed to align the superparamagnetic beads (of <math> 1 \mu m </math> size) within the vesicles in chains. Since the force to move the beads is proportional to the magnetic field gradient and it also has to be equal to the hydrodynamic drag force, the field to move beads of a certain radius a certain distance can be estimated.
+
A theoretical minimum magnetic field of <math> 59 \mu T </math> is needed to align the superparamagnetic beads (of <math> 1 \mu m </math> size) within the vesicles in chains. Since the force to move the vesicle containing beads is proportional to the magnetic field gradient and it also has to be equal to the hydrodynamic drag force, the necessary field can be estimated. The direction of the magnetic field gradient also determines the direction of movement of the vesicles. A rotating magnetic field causes the
 +
superparamagnetic chain to rotate inside the vesicle, eventually causing a rotation of the vesicle itself.
 +
A fluorescein was added continuous phase fluid to prove
 +
that the vesicle is not leaking any content. At
 +
high cons the fluorescence. When a vesicle is moved across the
 +
microchamber, repeated rotations of the chains were initiated
 +
without detection of any fluorescent signal, which shows
 +
that the vesicle is leakproof. But, adding the
 +
membrane-porating surfactant Triton-X causes
 +
water to permeate through the membrane and a strong increase in fluorescent
 +
signal can e observed (Fig. 3). Generally, after releasing the content of the vesicle, the intravesicular
 +
fluid volume mixes diffusively with the surrounding

Revision as of 03:24, 11 November 2010

Birgit Hausmann

Reference

T. Franke, L. Schmid, D. A. Weitz and A. Wixforth "Magneto-mechanical mixing and manipulation of picoliter volumes in vesicles" Lab Chip, 9, 2831-2835 2009

Keywords

Overview

Magnetic manipulation, positioning, agitation and mixing of ultrasmall liquid volumes has been realized utilizing superparamagnetic beads in giant unilamellar vesicles. In the presence of a magnetic field the beads align to form extended chains while a rotating magnetic field provokes the chains to break up into smaller fragments caused by the interplay of viscous friction and magnetic attraction.

Results and Discussion

Franke 1.jpg
Franke 2.jpg
Franke 3.jpg
Franke 5.jpg
Franke 6.jpg

While a magnetic field gradient generates a force on the magnetic dipole chains a rotational field introduces spinning. An electroformation method was used to fabricate the vesicles. The lipid in chloroform was deposited onto two indium tin oxide (ITO) coated glass slides and the organic solvent was evaporated in vacuum. An aqueous solution containing the superparamagnetic beads was added to the dried lipid. The two ITO plates were mounted in parallel and an electric field was applied. Finally, the voltage was increased to facilitate the separation of vesicles. A theoretical minimum magnetic field of <math> 59 \mu T </math> is needed to align the superparamagnetic beads (of <math> 1 \mu m </math> size) within the vesicles in chains. Since the force to move the vesicle containing beads is proportional to the magnetic field gradient and it also has to be equal to the hydrodynamic drag force, the necessary field can be estimated. The direction of the magnetic field gradient also determines the direction of movement of the vesicles. A rotating magnetic field causes the superparamagnetic chain to rotate inside the vesicle, eventually causing a rotation of the vesicle itself. A fluorescein was added continuous phase fluid to prove that the vesicle is not leaking any content. At high cons the fluorescence. When a vesicle is moved across the microchamber, repeated rotations of the chains were initiated without detection of any fluorescent signal, which shows that the vesicle is leakproof. But, adding the membrane-porating surfactant Triton-X causes water to permeate through the membrane and a strong increase in fluorescent signal can e observed (Fig. 3). Generally, after releasing the content of the vesicle, the intravesicular fluid volume mixes diffusively with the surrounding