Difference between revisions of "Magneto-mechanical mixing and manipulation of picoliter volumes in vesicles"
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== Overview == | == Overview == | ||
| − | Magnetic manipulation, positioning, agitation and mixing of ultrasmall liquid volumes has been realized utilizing superparamagnetic beads in giant unilamellar vesicles. In the | + | Magnetic manipulation, positioning, agitation and mixing of ultrasmall liquid volumes has been realized utilizing superparamagnetic beads in giant unilamellar vesicles. In the presence of a magnetic field the beads align to form |
extended chains while a rotating magnetic field provokes the chains to break up into smaller fragments caused by the | extended chains while a rotating magnetic field provokes the chains to break up into smaller fragments caused by the | ||
interplay of viscous friction and magnetic attraction. | interplay of viscous friction and magnetic attraction. | ||
| Line 14: | Line 14: | ||
== Results and Discussion == | == Results and Discussion == | ||
[[Image:Franke 1.jpg|400px|thumb|left|]] [[Image:Franke 2.jpg|400px|thumb|right|]] [[Image:Franke 3.jpg|900px|thumb|left|]] [[Image:Franke 5.jpg|400px|thumb|left|]][[Image:Franke 6.jpg|400px|thumb|right|]] | [[Image:Franke 1.jpg|400px|thumb|left|]] [[Image:Franke 2.jpg|400px|thumb|right|]] [[Image:Franke 3.jpg|900px|thumb|left|]] [[Image:Franke 5.jpg|400px|thumb|left|]][[Image:Franke 6.jpg|400px|thumb|right|]] | ||
| + | While a magnetic field gradient generates a force on the magnetic dipole chains a rotational field introduces spinning. | ||
| + | An electroformation method was used to fabricate the vesicles. The lipid in chloroform was deposited onto two indium tin oxide (ITO) coated glass slides and the organic solvent was evaporated in vacuum. An aqueous solution | ||
| + | containing the superparamagnetic beads was added to the dried lipid. The two ITO plates were mounted in parallel and an electric field was applied. Finally, the voltage was increased to facilitate the separation | ||
| + | of vesicles. | ||
Revision as of 03:06, 11 November 2010
Birgit Hausmann
Reference
Keywords
Overview
Magnetic manipulation, positioning, agitation and mixing of ultrasmall liquid volumes has been realized utilizing superparamagnetic beads in giant unilamellar vesicles. In the presence of a magnetic field the beads align to form extended chains while a rotating magnetic field provokes the chains to break up into smaller fragments caused by the interplay of viscous friction and magnetic attraction.
Results and Discussion
While a magnetic field gradient generates a force on the magnetic dipole chains a rotational field introduces spinning. An electroformation method was used to fabricate the vesicles. The lipid in chloroform was deposited onto two indium tin oxide (ITO) coated glass slides and the organic solvent was evaporated in vacuum. An aqueous solution containing the superparamagnetic beads was added to the dried lipid. The two ITO plates were mounted in parallel and an electric field was applied. Finally, the voltage was increased to facilitate the separation of vesicles.
