Difference between revisions of "FRET"

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[[image:FRET3.png]]
 
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Figure 1. Schematic of FRET between the donor chromophore, cyan fluorescent protein (CFP), and the acceptor chromophore, yellow fluorescent protein (YFP). FRET will only occur when the two chromophores are sufficiently close to each other.
 
Figure 1. Schematic of FRET between the donor chromophore, cyan fluorescent protein (CFP), and the acceptor chromophore, yellow fluorescent protein (YFP). FRET will only occur when the two chromophores are sufficiently close to each other.
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==Theoretical Basis==
 
==Theoretical Basis==
  

Revision as of 23:28, 4 December 2011

Contributed by Daniel Daniel

Introduction

Förster resonance energy transfer (FRET) is a mechanism that describes the transfer of energy between chromophores. A donor chromophore, which is in its electronically excited state, can transfer the energy non-radiatively to a acceptor chromophore that is nearby (typically less than 10 nm away) through dipole-dipole coupling. This process is known as FRET and it is exquisitely dependent on the distance between the two chromophores. FRET is often referred to as fluorescence resonance energy transfer, though this is a misnomer since the energy transfer is non-radiative and does not involve fluorescence. FRET allows imaging of objects that is separated by a distance of the order of 10 nm, which is way below the abbe diffraction limit of a microscope of 200 nm. It is one of the modern imaging techniques that allows us to surpass the diffraction limit that is characteristic of wide-field microscopy.


FRET3.png

Figure 1. Schematic of FRET between the donor chromophore, cyan fluorescent protein (CFP), and the acceptor chromophore, yellow fluorescent protein (YFP). FRET will only occur when the two chromophores are sufficiently close to each other.


Theoretical Basis

FRET2.jpg

Applications

FRET1.jpg

Acknowledgements

Figures 2 and 3 are taken from http://www.olympusfluoview.com/applications/fretintro.html

Keyword in references:

Crosslinking of cell-derived 3D scaffolds up-regulates the stretching and unfolding of new extracellular matrix assembled by reseeded cells

FRET and FCS Imaging Techniques