Digital Holographic Microscopy for 3D Imaging of Complex Fluids and Biological Systems

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Written by: Grant England AP225 Fall, 2011

Info

Title: Digital Holographic Microscopy for 3D Imaging of Complex Fluids and Biological Systems

Authors: Vinothan N. Manoharan

@Harvard: http://books.nap.edu/openbook.php?record_id=12821&page=5

Summary

In this paper, a method for resolving three dimensional objects, at a timescale much faster than that achieved with confocal microscopy and for a much lower cost, is described. By using Fourier transformations and other mathematics, a three dimensional representation of a structure can be back-calculated by using the interference pattern generated by a laser shining through the sample. So, using this method coupled with a digital camera which can capture images at 30 fps, 3D reconstructions of particles in solution or other interesting materials can be created.


Methods and Results

HologramF1.jpg


Conclusions

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