Biopolymer network geometries: Characterization, regeneration, and elastic properties

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Introduction

Researchers in George Whitesides and David Weitz's groups designed a model system of a polymer network with active constituents to explore the way that cells control their mechanical properties. Cells use networks composed of active filaments controlled by molecular motors. Cells use networks composed of active filaments controlled by molecular motors. The system in question is a filamentous actin (F-actin) network, made active via myosin molecular motors anchored by the cross-linking agent filamin A (FLNa). By inducing internal stress in the network, researchers were able to manipulate the elastic properties of the gel over 3 orders of magnitude. It has been demonstrated that systems containing only a few percent protein by volume can experience a transition from liquid-like to solid-like mechanical behavior. This fact, coupled with the tuning of active components opens up the possibility of active biomaterials that adapt mechanically to their environment.

Results/Conclusions

A number of properties of the system were explored. Some key findings are listed below:

FLNa crosslinking only increased stiffness slightly. According to the researchers, the relative softness, or "floppiness" of the crosslinks caused it to dominate the mechanical behavior in the linear regime. Once myosin II thick filaments were included in the network, the stiffness and the ratio of loss to storage decreased, associated with more solid-like behavior. blebbistatin was used to de-activate the myosin motors, and all effect on stiffness disappeared. A slight increase in loss with increasing concentration was noted. Local alignment is present without or with FLNa, but the long-range structure has no alignment. When myosin bond were permanent (formed in an ATP-free environment), significant bundling and alignment was noted. Both FLNa and myosin are required for local "bundling", or contraction. For the highest stress values, the ratio of the loss modulus to the storage modulus increased slightly for the active networks for frequencies below 0.2 rad/s, while passive networks showed frequency-independence of the loss modulus in high external stress states. These results imply that stress relaxation is mediated by a process with a characteristic time scale of larger than 5 seconds--a time scale consistent with measured myosin motor release rates. The Myosin motor activity can make actin networks behave more like solid by increasing the internal stress. Effect of tension sensitivity and unbinding 1. Myosin motors must be active 2. f-actin cross-linkers are required as anchor points, otherwise the myosin motors permit sliding One order of magnitude lower elastic moduli, possibly due to filament length (dependence supported by these experiments) Specific alignment or ordering might not be required to see stress stiffening.