Biofilms as complex fluids

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Entry by Emily Redston, AP 225, Fall 2011

Work in progress

Reference

Biofilms as Complex Fluids by J. N. Wilking, T. E. Angelini, A. Seminara, M. P. Brenner, and D. A. Weitz. MRS Bulletin, 26, 385-391 (2011)

Introduction

Bacterial biofilms can be found on nearly every surface as long as there is moisture and nutrients. They can have a positive impact in areas such as water treatment and waste sequestration, but they also play a devastating role in many bacteria-related problems like tooth decay and hospital-acquired infections. A better understanding of the structure, mechanics, and dynamics of biofilms is necessary for both their removal and for the optimization of their properties.

Viewing biofilms as a complex fluid is a good starting point for analyzing their structure and properties. A bioflim can be seen as a composite of colloids (bacterial cells) embedded in a cross-linked polymer gel (extracellular matrix -- ECM).

Biofilm Structure

Bacterial cells are rigid and have well-defined shapes like spheres or rods. Since the bacteria within a biofilm are mostly sessile and cannot generate forces outside of the cell, they control the structural and mechanical properties of the biofilm by regulating the composition of the ECM. The ECM is primarily composed of polysaccharides cross-linked by proteins and multivalent cations. This matrix provides the scaffold that holds the bacteria together; it gives the biofilm its mechanical integrety.

Viscoelasticity

Spatial Gradients

Conclusion