A high-throughput capillary assay for bacterial chemotaxis

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Zach Wissner-Gross (March 2, 2009)

Information

A high-throughput capillary assay for bacterial chemotaxis

Russell Bainer, Heungwon Park, and Phillipe Cluzel

Journal of Microbiological Methods, 2003, 55, 315-319

Soft matter Keywords

E. coli, chemotaxis, capillary

Summary

A methods paper, this article is relatively straightforward. The authors use a 96-well plate to perform a high-throughput assay for bacterial chemotaxis by setting up an array of gradients of L-aspartate (a chemoattractant). They establish these gradients using capillary tubes: first they dipped the tubes into motility medium either with or without the L-aspartate. They then sealed the tubes from above with wax, and finally they resuspended the tubes in 96-well plates inoculated with various bacterial concentrations.