Difference between revisions of "A high-throughput capillary assay for bacterial chemotaxis"

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==Summary==
 
==Summary==
  
A methods paper, this article is relatively straightforward. The authors use a 96-well plate to perform a high-throughput assay for bacterial chemotaxis by setting up an array of gradients of L-aspartate (a chemoattractant). They establish these gradients using capillary tubes: first they dipped the tubes into motility medium either with or without the L-aspartate. They then sealed the tubes from above with wax, and finally they resuspended the tubes in 96-well plates inoculated with various concentrations of bacteria (<i> E. coli</i>).
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A methods paper, this article is relatively straightforward. The authors use a 96-well plate to perform a high-throughput assay for bacterial chemotaxis by setting up an array of gradients of L-aspartate (a chemoattractant). They establish these gradients using capillary tubes: first they dipped the tubes into motility medium either with or without the L-aspartate. They then sealed the tubes from above with wax, and finally they resuspended the tubes in 96-well plates inoculated with various concentrations of bacteria (<i>E. coli</i>).

Revision as of 22:03, 1 March 2009

Zach Wissner-Gross (March 2, 2009)

Information

A high-throughput capillary assay for bacterial chemotaxis

Russell Bainer, Heungwon Park, and Phillipe Cluzel

Journal of Microbiological Methods, 2003, 55, 315-319

Soft matter Keywords

E. coli, chemotaxis, capillary

Summary

A methods paper, this article is relatively straightforward. The authors use a 96-well plate to perform a high-throughput assay for bacterial chemotaxis by setting up an array of gradients of L-aspartate (a chemoattractant). They establish these gradients using capillary tubes: first they dipped the tubes into motility medium either with or without the L-aspartate. They then sealed the tubes from above with wax, and finally they resuspended the tubes in 96-well plates inoculated with various concentrations of bacteria (E. coli).