A Blind Spot in Confocal Reflection Microscopy: The Dependence of Fiber Brightness on Fiber Orientation in Imaging Biopolymer Networks
Entry by Leon Furchtgott, APP 225 Fall 2010.
A Blind Spot in Confocal Reflection Microscopy: The Dependence of Fiber Brightness on Fiber Orientation in Imaging Biopolymer Networks. L.M. Jawerth, S. Munster, D.A. Vader, B. Fabry, and D.A. Weitz. (2010). Biophysical Journal, 98, L01-L03.
The paper is interested in imaging techniques for biopolymers. In particular, the paper compares the effectiveness in imaging collagen networks of two confocal imaging methods, confocal reflection microscopy (CRM) and confocal fluorescence microscopy (CFM). The authors simultaneously image collagen using the two techniques and find that in CRM, fiber brightness depends strongly on fiber orientation. This explains why when using CRM to image collagen, the network appears to be aligned with the imaging plane, whereas in CFM, the network seems isotropic.
Difference between CRM and CFM:
Previous research in collagen network architecture:
Relation to Soft Matter
This paper gives insight into a more experimental area of soft-matter physics than what we covered in our discussions of polymers. In particular it shows the great sensitivity of results about biopolymers to the imaging technique used and the dangers in using the wrong imaging technique for looking at polymers.