Vapor Diffusion Method

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The vapor diffusion method is a common technique used to crystallize proteins. There are two different types of techniques within this category: hanging drop and sitting drop methods. Both of them consist of having a drop with protein solution situated either on top (hanging drop) or next to (sitting drop) a reservoir containing precipitant (e.g. a concentrated salt solution). As the water from the protein solution vaporizes, it transfers to the reservoir and crystallization occurs.


Fig. 1 Hanging drop method.


Fig. 2 Sitting drop method.